DNA

Part:BBa_K3791025:Design

Designed by: Laura Sánchez Ruiz, Auba Fuster Palà   Group: iGEM21_UPF_Barcelona   (2021-10-12)


Construct for modulating protein E transcription


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The assembling of this construct was carried out considering the logic order of transcriptional regulatory elements. That is: the promoter regulatory sequences on the 5’ end, followed by the RBS (ribosome binding site), the gene of interest, and the His operon terminator on the 3’ end.


Source

All of the elements listed for the formation of this construct were obtained from the Parts Registry: pBAD AraC promoter (BBa_I0500), RBS (BBa_K2306014), Protein E (BBa_K2500006) and His operon terminator (BBa_B0053).